Detection of bak/bax activating conformation change by intracellular flow cytometry
- Author(s)
- Dewson, G;
- Details
- Publication Year 2015-05-01,Volume 2015,Issue #5,Page 477-480
- Journal Title
- Cold Spring Harb Protoc
- Publication Type
- Journal Article
- Abstract
- Like the commonly used immunoprecipitation (IP) approach, this procedure for the detection of activated Bak or Bax by intracellular flow cytometry is based on the principle that Bak and Bax, during activation, expose occluded amino-terminal epitopes that can be recognized by conformation-specific antibodies. Flow cytometric analysis requires fewer cells and is less time-consuming than IP. Further, in contrast to IP, flow cytometry produces a quantifiable assessment of the percentage of cells containing activated Bak or Bax, which can be correlated with cell death.
- Publisher
- CSHLP
- Research Division(s)
- Cell Signalling And Cell Death
- PubMed ID
- 25934938
- Publisher's Version
- https://doi.org/10.1101/pdb.prot086462
- NHMRC Grants
- NHMRC/637337,
- ARC Grants
- ARC/FT100100791,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2015-05-22 11:19:35
Last Modified: 2016-01-21 08:49:03