Blue native PAGE and antibody gel shift to assess bak and bax conformation change and oligomerization
- Author(s)
- Dewson, G;
- Details
- Publication Year 2015-05-01,Volume 2015,Issue #5,Page 485-489
- Journal Title
- Cold Spring Harb Protoc
- Publication Type
- Journal Article
- Abstract
- Blue native PAGE (BN-PAGE) uses Coomassie dye rather than denaturing SDS to provide a negative charge to proteins for electrophoresis. As such, it is a useful assay for investigating native supramolecular membrane complexes without the need for cross-linking. As Bak and Bax oligomers form in the mitochondrial outer membrane, and they can be efficiently monitored by BN-PAGE. Furthermore, BN-PAGE performed in conjunction with gel-shift using conformation-specific antibodies can provide additional information regarding the activation state of Bak or Bax in specific membrane complexes.
- Publisher
- CSHLP
- Research Division(s)
- Cell Signalling And Cell Death
- PubMed ID
- 25934940
- Publisher's Version
- https://doi.org/10.1101/pdb.prot086488
- NHMRC Grants
- NHMRC/637335,
- ARC Grants
- ARC/FT100100791,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2015-05-22 11:19:35
Last Modified: 2016-01-21 08:48:06