Mesodermal expression of Moz Is necessary for cardiac septum development
- Author(s)
- Vanyai, HK; Thomas, T; Voss, AK;
- Details
- Publication Year 2015-07-01,Volume 403,Issue #1,Page 22-9
- Journal Title
- Dev Biol
- Publication Type
- Journal Article
- Abstract
- Ventricular septal defects (VSDs) are the most commonly occurring congenital heart defect. They are regularly associated with complex syndromes, including DiGeorge syndrome and Holt-Oram syndrome, which are characterised by haploinsufficiency for the T-box transcription factors TBX1 and TBX5, respectively. The histone acetyltransferase monocytic leukaemia zinc finger protein, MOZ (MYST3/KAT6A), is required for the expression of the Tbx1 and Tbx5 genes. Homozygous loss of MOZ results in DiGeorge syndrome-like defects including VSD. The Moz gene is expressed in the ectodermal, mesodermal and endodermal aspects of the developing pharyngeal apparatus and heart, however it is unclear in which of these tissues MOZ is required for heart development. The role of MOZ in the activation of Tbx1 would suggest a requirement for MOZ in the mesoderm, because deletion of Tbx1 in the mesoderm causes VSDs. Here, we investigated the tissue-specific requirements for MOZ in the mesoderm. We demonstrate that Mesp1-cre-mediated deletion of Moz results in high penetrance of VSDs and overriding aorta and a significant decrease in MOZ-dependent Tbx1 and Tbx5 expression. Together, our data suggest that the molecular pathogenesis of VSDs in Moz germline mutant mice is due to loss of MOZ-dependent activation of mesodermal Tbx1 and Tbx5 expression.
- Publisher
- Elsevier
- Research Division(s)
- Development And Cancer
- PubMed ID
- 25912687
- Publisher's Version
- https://doi.org/10.1016/j.ydbio.2015.04.011
- NHMRC Grants
- NHMRC/1010851, NHMRC/1008699, NHMRC/1051078, NHMRC/1003435, NHMRC/575512,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2015-05-20 09:04:11
Last Modified: 2015-07-14 02:34:41