A step beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
Details
Publication Year 2014, Volume May 23, Issue #87, Page 51549
Journal Title
J Vis Exp
Publication Type
Journal Article
Abstract
Fluorescence by Unbound Excitation from Luminescence (FUEL) is a radiative excitation-emission process that produces increased signal and contrast enhancement in vitro and in vivo. FUEL shares many of the same underlying principles as Bioluminescence Resonance Energy Transfer (BRET), yet greatly differs in the acceptable working distances between the luminescent source and the fluorescent entity. While BRET is effectively limited to a maximum of 2 times the Forster radius, commonly less than 14 nm, FUEL can occur at distances up to microm or even cm in the absence of an optical absorber. Here we expand upon the foundation and applicability of FUEL by reviewing the relevant principles behind the phenomenon and demonstrate its compatibility with a wide variety of fluorophores and fluorescent nanoparticles. Further, the utility of antibody-targeted FUEL is explored. The examples shown here provide evidence that FUEL can be utilized for applications where BRET is not possible, filling the spatial void that exists between BRET and traditional whole animal imaging.
Publisher
JOVE
WEHI Research Division(s)
Systems Biology And Personalised Medicine
PubMed ID
24894759
Publisher's Version
https://doi.org/10.3791/51549
Rights Notice
Refer to copyright notice on published article.


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Last Modified: 2015-05-20 11:44:07
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