CD3 signals on gammadelta T cells identify IL-17A-producing Vgamma6Vdelta1 T cells
Journal Title
Immunol Cell Biol
Publication Type
Journal Article in press
Abstract
Interleukin-17A (IL-17A) is a pro-inflammatory cytokine that has an important role at mucosal sites in a wide range of immune responses including infection, allergy and auto-immunity. gammadelta T cells are recognized as IL-17 producers, but based on the level of CD3 expression, we now define the remarkable ability of a CD3bright gammadelta T-cell subset with an effector memory phenotype to rapidly produce IL-17A, but not interferon-gamma. CD3bright gammadelta T cells uniformly express the canonical germline encoded Vgamma6/Vdelta1+ T-cell receptor. They are widely distributed with a preferential representation in the lungs and skin are negatively impacted in the absence of retinoic acid receptor-related orphan receptor gammat expression or endogenous flora. This population responded rapidly to various stimuli in a mechanism involving IL-23 and NOD-like receptor family, pyrin domain containing 3 (NLRP3)-inflammasome-dependent IL-1beta. Finally, we demonstrated that IL-17-producing CD3bright gammadelta T cells responded promptly and strongly to pneumococcal infection and during skin inflammation. Here, we propose a new way to specifically analyze IL-17-producing Vgamma6/Vdelta1+ T cells based on the level of CD3 signals. Using this gating strategy, our data reinforce the crucial role of this gammadelta T-cell subset in respiratory and skin disorders.Immunology and Cell Biology advance online publication, 11 November 2014; doi:10.1038/icb.2014.94.
Publisher
NPG
Research Division(s)
Molecular Immunology
Terms of Use/Rights Notice
© 2014 Australasian Society for Immunology


Creation Date: 2014-11-14 01:51:47
Last Modified: 2018-07-09 03:09:36
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