IL-10 regulates Aicda expression through miR-155
- Author(s)
- Fairfax, KA; Gantier, MP; Mackay, F; Williams, BR; McCoy, CE;
- Details
- Publication Year 2015-01,Volume 97,Issue #1,Page 71-78
- Journal Title
- J Leukoc Biol
- Publication Type
- Journal Article
- Abstract
- Aicda is a critical component of antibody class-switching in B cells. In this work, we study the impact of TLR4 activation and IL-10 stimulation on Aicda expression in B cells. Through the global analysis of miRNAs in response to TLR4 activation, in combination with IL-10 stimulation, we identified that IL-10 can suppress TLR4-induced miR-155 expression, an effect that resulted in enhanced Aicda expression. Furthermore, when preventing miR-155 control of Aicda expression, by genetic mutation of its target site in the Aicda mRNA, IL-10 could further potentiate Aicda expression. Given that miR-155 expression is lost, and expression levels of both Aicda and IL-10 are high in diseases, such as Burkitt's lymphoma, our results suggest a stringent and sophisticated control of Aicda by a novel IL-10/miR-155 axis, where the imbalance of IL-10 and/or miR-155 may contribute to disease pathogenesis.
- Publisher
- Society for Leukocyte Biology
- Keywords
- AID; B cell; TLR4; antibody class-switching; interleukin; miRNA
- Research Division(s)
- Molecular Medicine
- PubMed ID
- 25381386
- Publisher's Version
- https://doi.org/10.1189/jlb.2A0314-178R
- NHMRC Grants
- NHMRC/516786,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2014-11-14 01:51:47
Last Modified: 2016-02-18 02:00:38