Single-cell gene expression profiles define self-renewing, pluripotent, and lineage primed states of human pluripotent stem cells
- Details
- Publication Year 2014-06-03,Volume 2,Issue #6,Page 881-95
- Journal Title
- Stem Cell Reports
- Publication Type
- Journal Article
- Abstract
- Pluripotent stem cells display significant heterogeneity in gene expression, but whether this diversity is an inherent feature of the pluripotent state remains unknown. Single-cell gene expression analysis in cell subsets defined by surface antigen expression revealed that human embryonic stem cell cultures exist as a continuum of cell states, even under defined conditions that drive self-renewal. The majority of the population expressed canonical pluripotency transcription factors and could differentiate into derivatives of all three germ layers. A minority subpopulation of cells displayed high self-renewal capacity, consistently high transcripts for all pluripotency-related genes studied, and no lineage priming. This subpopulation was characterized by its expression of a particular set of intercellular signaling molecules whose genes shared common regulatory features. Our data support a model of an inherently metastable self-renewing population that gives rise to a continuum of intermediate pluripotent states, which ultimately become primed for lineage specification.
- Publisher
- Cell Press
- Research Division(s)
- Molecular Medicine
- Link To PubMed Central Version
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050352/
- Publisher's Version
- https://doi.org/10.1016/j.stemcr.2014.04.014
- Terms of Use/Rights Notice
- Copyright © 2014 The Authors. Published by Elsevier Inc.
Creation Date: 2014-12-19 11:44:53
Last Modified: 2014-12-19 11:57:18