Oct4-GFP expression during transformation of gonocytes into spermatogonial stem cells in the perinatal mouse testis
- Author(s)
- Li, R; Vannitamby, A; Zhang, JG; Fehmel, EL; Southwell, BR; Hutson, JM;
- Details
- Publication Year 2015-12,Volume 50,Issue #12,Page 2084-2089
- Journal Title
- J Pediatr Surg
- Publication Type
- Journal Article
- Abstract
- BACKGROUND/AIM: In cryptorchidism perinatal failure to switch off Oct4, a germ cell (GC) marker, may lead to carcinoma in situ. We aimed to analyze Oct4 expression during mouse gonocyte transformation into spermatogonial stem cells (SSC). MATERIALS AND METHODS: Testes from OG2 (Oct4-promoter driven eGFP) mice at embryonic day (E) 17 and postnatal day P0-10 underwent immunohistochemistry and immunoblotting. Antibodies against MVH, AMH, Ki67, and c-Kit were visualized by confocal microscopy. Numbers of Oct4-GFP+ GC and Oct4-GFP- GC/tubule were counted using ImageJ. Data were analyzed using nonparametric one-way ANOVA. RESULTS: GC from E17-P4 were Oct4-GFP+. Numbers of Oct4-GFP- GC/tubule increased from P6-10, whereas Oct4-GFP+ GC/tubule numbers remained similar between P6 and P10. Sertoli cells proliferated from E17-P10, whereas GC only proliferated from P2. Gonocytes (Oct4-GFP+/c-Kit-) central in tubules migrated to the basement membrane to become prospermatogonia (Oct4-GFP+/c-Kit-) and then SSC (Oct4-GFP+/c-Kit+) from day 4 and further developed into Oct4-GFP-/c-Kit+ at P6. CONCLUSION: In Oct4-GFP mice both centrally located gonocytes and prospermatogonia located at the tubular basement membrane were Oct4-GFP+/c-Kit- before further developing into SSC (Oct4-GFP+/c-Kit+). This indicates that Oct4 is important in gonocyte transformation into SSC. Understanding this process will aid GC tumor diagnostics and fertility potential in boys with UDT undergoing orchidopexy.
- Publisher
- Elsevier
- Research Division(s)
- Cancer And Haematology
- PubMed ID
- 26386877
- Publisher's Version
- https://doi.org/10.1016/j.jpedsurg.2015.08.031
- NHMRC Grants
- NHMRC/1049014, NHMRC/1016647,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2015-09-24 02:12:25
Last Modified: 2016-01-13 09:50:37