G ganglioside and phosphatidylethanolamine-containing lipids are adipose tissue markers of insulin resistance in obese women
- Author(s)
- Wentworth, JM; Naselli, G; Ngui, K; Smyth, GK; Liu, R; O'Brien, PE; Bruce, C; Weir, J; Cinel, M; Meikle, PJ; Harrison, LC;
- Details
- Publication Year 2016-04,Volume 40,Issue #4,Page 706-13
- Journal Title
- Int J Obes
- Publication Type
- Journal Article
- Abstract
- AimsThe association between central obesity and insulin resistance reflects the properties of visceral adipose tissue. Our aim was to gain further insight into this association by analysing the lipid composition of subcutaneous and omental adipose tissue in obese women with and without insulin resistance.MethodsSubcutaneous and omental adipose tissue and serum were obtained from 29 obese non-diabetic women, 13 of whom were hyperinsulinemic. Histology, and lipid and gene profiling were performed.ResultsIn omental adipose tissue of obese, insulin-resistant women, adipocyte hypertrophy and macrophage infiltration were accompanied by an increase in GM3 ganglioside and its synthesis enzyme ST3GAL5; in addition, phosphatidylethanolamine (PE) lipids were increased and their degradation enzyme, PEMT, decreased. ST3GAL5 was expressed predominantly in adipose stromovascular cells and PEMT in adipocytes. Insulin resistance was also associated with an increase in PE lipids in serum.InterpretationThe relevance of these findings to insulin resistance in humans is supported by published mouse studies in which adipocyte GM3 ganglioside, increased by the inflammatory cytokine tumour necrosis factor-alpha, impaired insulin action, and PEMT was required for adipocyte lipid storage. Thus, in visceral adipose tissue of obese humans, an increase in GM3 ganglioside secondary to inflammation may contribute to insulin resistance and a decrease in PEMT may be a compensatory response to adipocyte hypertrophy.International Journal of Obesity accepted article preview online, 26 October 2015. doi:10.1038/ijo.2015.223.
- Publisher
- NPG
- Research Division(s)
- Population Health And Immunity; Bioinformatics
- PubMed ID
- 26499445
- Publisher's Version
- https://doi.org/10.1038/ijo.2015.223
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2015-10-28 01:52:45
Last Modified: 2019-04-01 08:56:16