Bak deletion stimulates gastric epithelial proliferation and enhances Helicobacter felis-induced gastric atrophy and dysplasia in mice

Duckworth, CA; Abuderman, AA; Burkitt, MD; Williams, JM; O&#39;Reilly, LA; Pritchard, DM

Author(s): Duckworth, CA; Abuderman, AA; Burkitt, MD; Williams, JM; O'Reilly, LA; Pritchard, DM;
Details: Publication Year 2015-09-15,Volume 309,Issue #6,Page G420-30
Journal Title: Am J Physiol Gastrointest Liver Physiol
Publication Type: Journal Article
Abstract: Helicobacter infection causes a chronic superficial gastritis that in some cases progresses via atrophic gastritis to adenocarcinoma. Proapoptotic bak has been shown to regulate radiation-induced apoptosis in the stomach and colon and also susceptibility to colorectal carcinogenesis in vivo. Therefore we investigated the gastric mucosal pathology following H. felis infection in bak-null mice at 6 or 48 wk postinfection. Primary gastric gland culture from bak-null mice was also used to assess the effects of bak deletion on IFN-gamma-, TNF-alpha-, or IL-1beta-induced apoptosis. bak-null gastric corpus glands were longer, had increased epithelial Ki-67 expression, and contained fewer parietal and enteroendocrine cells compared with the wild type (wt). In wt mice, bak was expressed at the luminal surface of gastric corpus glands, and this increased 2 wk post-H. felis infection. Apoptotic cell numbers were decreased in bak-null corpus 6 and 48 wk following infection and in primary gland cultures following cytokine administration. Increased gastric epithelial Ki-67 labeling index was observed in C57BL/6 mice after H. felis infection, whereas no such increase was detected in bak-null mice. More severe gastric atrophy was observed in bak-null compared with C57BL/6 mice 6 and 48 wk postinfection, and 76% of bak-null compared with 25% of C57BL/6 mice showed evidence of gastric dysplasia following long-term infection. Collectively, bak therefore regulates gastric epithelial cell apoptosis, proliferation, differentiation, mucosal thickness, and susceptibility to gastric atrophy and dysplasia following H. felis infection.
Publisher: APS
Research Division(s): Molecular Genetics Of Cancer
PubMed ID: 26159699
Link To PubMed Central Version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4572407/
Publisher's Version: https://doi.org/10.1152/ajpgi.00404.2014
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2015-10-26 12:12:50

Last Modified: 2015-10-28 01:42:49