Localization of dipeptidyl peptidase-4 (CD26) to human pancreatic ducts and islet alpha cells

Augstein, P; Naselli, G; Loudovaris, T; Hawthorne, WJ; Campbell, P; Bandala-Sanchez, E; Rogers, K; Heinke, P; Thomas, HE; Kay, TW; Harrison, LC

Author(s): Augstein, P; Naselli, G; Loudovaris, T; Hawthorne, WJ; Campbell, P; Bandala-Sanchez, E; Rogers, K; Heinke, P; Thomas, HE; Kay, TW; Harrison, LC;
Details: Publication Year 2015-12,Volume 110,Issue #3,Page 291-300
Journal Title: Diabetes Res Clin Pract
Publication Type: Journal Article
Abstract: AIM: DPP-4/CD26 degrades the incretins GLP-1 and GIP. The localization of DPP-4 within the human pancreas is not well documented but is likely to be relevant for understanding incretin function. We aimed to define the cellular localization of DPP-4 in the human pancreas from cadaveric organ donors with and without diabetes. METHODS: Pancreas was snap-frozen and immunoreactive DPP-4 detected in cryosections using the APAAP technique. For co-localization studies, pancreas sections were double-stained for DPP-4 and proinsulin or glucagon and scanned by confocal microscopy. Pancreata were digested and cells in islets and in islet-depleted, duct-enriched digests analyzed for expression of DPP-4 and other markers by flow cytometry. RESULTS: DPP-4 was expressed by pancreatic duct and islet cells. In pancreata from donors without diabetes or with type 2 diabetes, DPP-4-positive cells in islets had the same location and morphology as glucagon-positive cells, and the expression of DPP-4 and glucagon overlapped. In donors with type 1 diabetes, the majority of residual cells in islets were DPP-4-positive. CONCLUSION: In the human pancreas, DPP-4 expression is localized to duct and alpha cells. This finding is consistent with the view that DPP-4 regulates exposure to incretins of duct cells directly and of beta cells indirectly in a paracrine manner.
Publisher: Elsevier
Research Division(s): Population Health And Immunity; Systems Biology And Personalised Medicine
PubMed ID: 26515908
Publisher's Version: https://doi.org/10.1016/j.diabres.2015.10.010
NHMRC Grants: NHMRC/637301,
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2015-11-09 12:06:45

Last Modified: 2019-04-01 08:57:55