Enzyme treatment of colony stimulating factor: evidence for a peptide component.
- Author(s)
- Stanley, ER; Metcalf, D;
- Details
- Publication Year 1971-06,Volume 49,Issue #3,Page 281-90
- Journal Title
- The Australian journal of experimental biology and medical science
- Publication Type
- Journal Article
- Abstract
- The effects of various proteolytic enzymes, glycosidases and phos- pholipases on the bone marrow colony stimulating factor from human urine have been investigated. Colony stimulating factor Was found to be comparatively resistant to inactivation by all of the enzymes tested with the exception of alpha-chymotrypsin (at low concentrations), subtilisin and ficin. When biological activity remained following enzyme treatment, no change could be detected in the behaviour of colony stimulating factor on polyacrylmide disc electro- phoresis, under conditions in which a sieving effect on colony stimulating factor migration was observed. The studies have indicated the existence of a peptide component of colony stimulating factor, its relative resistance to enzymatic proteolysis and its presence as a minor fraction of the total protein in the urine of normal humans.
- PubMed ID
- 5119761
- Publisher's Version
- https://doi.org/10.1038/icb.1971.28
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
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Last Modified: 2015-10-29 11:25:03