Haematological effects in mice of partially purified colony stimulating factor (CSF) prepared from human urine.
- Author(s)
- Metcalf, D; Stanley, ER;
- Details
- Publication Year 1971-10,Volume 21,Issue #4,Page 481-92
- Journal Title
- British journal of haematology
- Publication Type
- Journal Article
- Abstract
- Single injections of partially purified human urine preparations containing 10 000–20 000 units of colony stimulating factor (CSF) produced a monocytosis and polymorph leucocytosis in neonatal C57BL mice which was maximal at 48 hr. Adult C57BL mice developed a monocytosis, and although no polymorph leucocytosis developed, labelling studies suggested an increased rate of granulopoiesis. In adult mice the injected CSF preparations did not alter total bone-marrow cellularity but increased the percentage of primitive granulocytes and caused an increase in the number of in vitro colony-forming cells and granulocytic cluster-forming cells. The preparations also increased the number of bone-marrow cells able to initiate colony and cluster formation immediately after culture of the bone-marrow cells in agar. The results suggest that CSF in vivo may stimulate both granulopoiesis and monocyte formation in an action similar to its action in agar cultures of bone-marrow cells
- PubMed ID
- 5122671
- Publisher's Version
- https://doi.org/10.1111/j.1365-2141.1971.tb02709.x
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2015-07-23 08:59:24
Last Modified: 2015-10-30 11:36:07