Identification of cDC1- and cDC2-committed DC progenitors reveals early lineage priming at the common DC progenitor stage in the bone marrow

Schlitzer, A; Sivakamasundari, V; Chen, J; Sumatoh, HR; Schreuder, J; Lum, J; Malleret, B; Zhang, S; Larbi, A; Zolezzi, F; Renia, L; Poidinger, M; Naik, S; Newell, EW; Robson, P; Ginhoux, F

Author(s): Schlitzer, A; Sivakamasundari, V; Chen, J; Sumatoh, HR; Schreuder, J; Lum, J; Malleret, B; Zhang, S; Larbi, A; Zolezzi, F; Renia, L; Poidinger, M; Naik, S; Newell, EW; Robson, P; Ginhoux, F;
Details: Publication Year 2015-06-08,Volume 16,Issue #7,Page 718-728
Journal Title: Nat Immunol
Publication Type: Journal Article
Abstract: Mouse conventional dendritic cells (cDCs) can be classified into two functionally distinct lineages: the CD8alpha+ (CD103+) cDC1 lineage, and the CD11b+ cDC2 lineage. cDCs arise from a cascade of bone marrow (BM) DC-committed progenitor cells that include the common DC progenitors (CDPs) and pre-DCs, which exit the BM and seed peripheral tissues before differentiating locally into mature cDCs. Where and when commitment to the cDC1 or cDC2 lineage occurs remains poorly understood. Here we found that transcriptional signatures of the cDC1 and cDC2 lineages became evident at the single-cell level from the CDP stage. We also identified Siglec-H and Ly6C as lineage markers that distinguished pre-DC subpopulations committed to the cDC1 lineage (Siglec-H-Ly6C- pre-DCs) or cDC2 lineage (Siglec-H-Ly6C+ pre-DCs). Our results indicate that commitment to the cDC1 or cDC2 lineage occurs in the BM and not in the periphery.
Publisher: NPG
Research Division(s): Molecular Medicine
PubMed ID: 26054720
Publisher's Version: https://doi.org/10.1038/ni.3200
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2015-06-22 12:12:32

Last Modified: 2015-09-22 09:20:58