A Plasmodium falciparum bromodomain protein regulates invasion gene expression
Details
Publication Year 2015-06-10, Volume 17, Issue #6, Page 741-51
Journal Title
Cell Host Microbe
Publication Type
Journal Article
Abstract
During red-blood-cell-stage infection of Plasmodium falciparum, the parasite undergoes repeated rounds of replication, egress, and invasion. Erythrocyte invasion involves specific interactions between host cell receptors and parasite ligands and coordinated expression of genes specific to this step of the life cycle. We show that a parasite-specific bromodomain protein, PfBDP1, binds to chromatin at transcriptional start sites of invasion-related genes and directly controls their expression. Conditional PfBDP1 knockdown causes a dramatic defect in parasite invasion and growth and results in transcriptional downregulation of multiple invasion-related genes at a time point critical for invasion. Conversely, PfBDP1 overexpression enhances expression of these same invasion-related genes. PfBDP1 binds to acetylated histone H3 and a second bromodomain protein, PfBDP2, suggesting a potential mechanism for gene recognition and control. Collectively, these findings show that PfBDP1 critically coordinates expression of invasion genes and indicate that targeting PfBDP1 could be an invaluable tool in malaria eradication.
Publisher
Cell Press
WEHI Research Division(s)
Infection And Immunity
PubMed ID
26067602
ARC Grants
ARC/DP110100483,
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2015-06-22 12:12:32
Last Modified: 2015-06-22 02:46:23
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