Multipotent RAG1+ progenitors emerge directly from haemogenic endothelium in human pluripotent stem cell-derived haematopoietic organoids
- Author(s)
- Motazedian, A; Bruveris, FF; Kumar, SV; Schiesser, JV; Chen, T; Ng, ES; Chidgey, AP; Wells, CA; Elefanty, AG; Stanley, EG;
- Details
- Publication Year 2020-01,Volume 22,Issue #1,Page 60-73
- Journal Title
- Nature Cell Biology
- Publication Type
- Journal Article
- Abstract
- Defining the ontogeny of the human adaptive immune system during embryogenesis has implications for understanding childhood diseases including leukaemias and autoimmune conditions. Using RAG1:GFP human pluripotent stem cell reporter lines, we examined human T-cell genesis from pluripotent-stem-cell-derived haematopoietic organoids. Under conditions favouring T-cell development, RAG1+ cells progressively upregulated a cohort of recognized T-cell-associated genes, arresting development at the CD4+CD8+ stage. Sort and re-culture experiments showed that early RAG1+ cells also possessed B-cell, myeloid and erythroid potential. Flow cytometry and single-cell-RNA-sequencing data showed that early RAG1+ cells co-expressed the endothelial/haematopoietic progenitor markers CD34, VECAD and CD90, whereas imaging studies identified RAG1+ cells within CD31+ endothelial structures that co-expressed SOX17+ or the endothelial marker CAV1. Collectively, these observations provide evidence for a wave of human T-cell development that originates directly from haemogenic endothelium via a RAG1+ intermediate with multilineage potential.
- Publisher
- NPG
- Research Division(s)
- Blood Cells And Blood Cancer
- PubMed ID
- 31907413
- Publisher's Version
- https://doi.org/10.1038/s41556-019-0445-8
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2020-02-24 09:56:00
Last Modified: 2020-02-24 12:25:23