Benchmarking single cell RNA-sequencing analysis pipelines using mixture control experiments
- Author(s)
- Tian, L; Dong, X; Freytag, S; Le Cao, KA; Su, S; JalalAbadi, A; Amann-Zalcenstein, D; Weber, TS; Seidi, A; Jabbari, JS; Naik, SH; Ritchie, ME;
- Details
- Publication Year 2019-06,Volume 16,Issue #6,Page 479-487
- Journal Title
- Nature Methods
- Publication Type
- Journal Article
- Abstract
- Single cell RNA-sequencing (scRNA-seq) technology has undergone rapid development in recent years, leading to an explosion in the number of tailored data analysis methods. However, the current lack of gold-standard benchmark datasets makes it difficult for researchers to systematically compare the performance of the many methods available. Here, we generated a realistic benchmark experiment that included single cells and admixtures of cells or RNA to create 'pseudo cells' from up to five distinct cancer cell lines. In total, 14 datasets were generated using both droplet and plate-based scRNA-seq protocols. We compared 3,913 combinations of data analysis methods for tasks ranging from normalization and imputation to clustering, trajectory analysis and data integration. Evaluation revealed pipelines suited to different types of data for different tasks. Our data and analysis provide a comprehensive framework for benchmarking most common scRNA-seq analysis steps.
- Publisher
- Springer Nature
- Research Division(s)
- Epigenetics And Development; Immunology
- PubMed ID
- 31133762
- Publisher's Version
- https://doi.org/10.1038/s41592-019-0425-8
- NHMRC Grants
- NHMRC/1143163, NHMRC/1124812, NHMRC/1062820, NHMRC/1104924,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2019-06-14 09:37:01
Last Modified: 2019-06-14 11:37:34