CARD11 is dispensable for homeostatic responses and suppressive activity of peripherally-induced FOXP3+ regulatory T cells
Publication Year 2019-09, Volume 97, Issue #8, Page 740-752
Journal Title
Immunology and Cell Biology
Publication Type
Journal Article
FOXP3(+) regulatory T (Treg) cells are essential for immunological tolerance and immune homeostasis. Despite a great deal of interest in modulating their number and function for the treatment of autoimmune disease or cancer, the precise mechanisms that control the homeostasis of Treg cells remain unclear. We report a new ENU-induced mutant mouse, loco, with atopic dermatitis and Treg cell deficiency typical of Card11 loss-of-function mutants. Three distinct single nucleotide variants were found in the Card11 introns 2, 10 and 20 that cause the loss of CARD11 expression in these mutant mice. These mutations caused the loss of thymic-derived, NRP1(+) Treg cells in neonatal and adult loco mice, however residual peripherally-induced NRP1(-) Treg cells remained. These peripherally generated Treg cells could be expanded in vivo by administration of IL-2:anti-IL-2 complexes, indicating this key homeostatic signaling axis remained intact in CARD11-deficient Treg cells. Furthermore, these expanded Treg cells could mediate near normal suppression of activated, conventional CD4(+) T cells, suggesting that CARD11 is dispensable for Treg cell function. In addition to shedding light on the requirements for CARD11 in Treg cell homeostasis and function, these data reveal novel non-coding Card11 loss-of-function mutations that impair expression of this critical immune-regulatory protein. This article is protected by copyright. All rights reserved.
WEHI Research Division(s)
Immunology; Inflammation; Blood Cells And Blood Cancer
PubMed ID
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Creation Date: 2019-06-14 09:37:01
Last Modified: 2021-05-12 11:12:35
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