Clathrin and GRK2/3 inhibitors block delta opioid receptor internalization in myenteric neurons and inhibit neuromuscular transmission in the mouse colon

DiCello, JJ; Rajasekhar, P; Eriksson, EM; Saito, A; Gondin, AB; Veldhuis, NA; Canals, M; Carbone, SE; Poole, DP

Author(s): DiCello, JJ; Rajasekhar, P; Eriksson, EM; Saito, A; Gondin, AB; Veldhuis, NA; Canals, M; Carbone, SE; Poole, DP;
Details: Publication Year 2019-05-15,Volume 317,Issue #2,Page G79-G80
Journal Title: American Journal of Physiology Gastrointestinal and Liver Physiology
Publication Type: Journal Article
Abstract: Endocytosis is a major mechanism through which cellular signaling by G protein-coupled receptors (GPCRs) is terminated. However, recent studies demonstrate that GPCRs are internalized in an active state and continue to signal from within endosomes, resulting in effects on cellular function that are distinct to those arising at the cell surface. Endocytosis inhibitors are commonly used to define the importance of GPCR internalization for physiological and pathophysiological processes. Here we provide the first detailed examination of the effects of these inhibitors on neurogenic contractions of gastrointestinal smooth muscle; a key preliminary step to evaluate the importance of GPCR endocytosis for gut function. Inhibitors of clathrin-mediated endocytosis (Pitstop2: PS2) or GRK2/3-dependent phosphorylation (Takeda compound 101: Cmpd101), significantly reduced GPCR internalization. However, they also attenuated cholinergic contractions through different mechanisms. PS2 abolished contractile responses by colonic muscle to SNC80 and morphine, which strongly and weakly internalize delta and mu opioid receptors, respectively. PS2 did not affect the increased myogenic contractile activity following removal of an inhibitory neural influence (tetrodotoxin) but suppressed electrically-evoked neurogenic contractions. Ca(2+) signaling by myenteric neurons in response to exogenous ATP was unaffected by PS2, suggesting inhibitory actions on neurotransmitter release, rather than neurotransmission. In contrast, Cmpd101 attenuated contractions to the cholinergic agonist carbachol, indicating direct effects on smooth muscle. We conclude that although PS2 and Cmpd101 are effective blockers of GPCR endocytosis in enteric neurons, these inhibitors are unsuitable for the study of neurally-mediated gut function due to their inhibitory effects on neuromuscular transmission and smooth muscle contractility.
Publisher: APS
Research Division(s): Population Health And Immunity
PubMed ID: 31091149
Publisher's Version: https://doi.org/10.1152/ajpgi.00085.2019
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2019-06-14 09:36:57

Last Modified: 2019-08-08 02:43:44