Heteronuclear NMR spectroscopy of proteins encapsulated in cubic phase lipids
- Author(s)
- Meikle, TG; Sethi, A; Keizer, DW; Babon, JJ; Separovic, F; Gooley, PR; Conn, CE; Yao, S;
- Journal Title
- Journal of Magnetic Resonance
- Publication Type
- Journal Article
- Abstract
- Lipidic cubic phases, which form spontaneously via the self-assembly of certain lipids in an aqueous environment, are highly prospective nanomaterials with applications in membrane protein X-ray crystallography and drug delivery. Here we report (1)H-(15)N heteronuclear single/multiple quantum coherence (HSQC, HMQC) spectra of (15)N-enriched proteins encapsulated in inverse bicontinuous lipidic cubic phases obtained on a standard commercial high resolution NMR spectrometer at ambient temperature. (15)N-enriched proteins encapsulated in this lipidic cubic phase show: (i) no significant changes in tertiary structure, (ii) significantly reduced solvent chemical exchange of backbone amides, which potentially provides a novel concept for quantifying residue-specific hydration; and (iii) improved spectral sensitivity achieved with band-selective excitation short-transient (BEST) spectroscopy, which is attributed to the presence of an abundant source of (1)H nuclear spins originating from the lipid component of the cubic phase.
- Publisher
- Elsevier
- Research Division(s)
- Structural Biology
- PubMed ID
- 31284168
- Publisher's Version
- https://doi.org/10.1016/j.jmr.2019.06.017
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2019-07-17 01:31:56
Last Modified: 2019-07-17 02:04:05