Vaccine-induced carbohydrate-specific memory B Cells reactivate during rodent malaria infection
- Author(s)
- Joseph, H; Tan, QY; Mazhari, R; Eriksson, EM; Schofield, L;
- Journal Title
- Frontiers in Immunology
- Publication Type
- Journal Article
- Abstract
- A long-standing challenge in malaria is the limited understanding of B cell immunity, previously hampered by lack of tools to phenotype rare antigen-specific cells. Our aim was to develop a method for identifying carbohydrate-specific B cells within lymphocyte populations and to determine whether a candidate vaccine generated functional memory B cells (MBCs) that reactivated upon challenge with Plasmodium (pRBCs). To this end, a new flow cytometric probe was validated and used to determine the kinetics of B cell activation against the candidate vaccine glycosylphosphatidylinositol conjugated to Keyhole Limpet Haemocyanin (GPI-KLH). Additionally, immunized C57BL/6 mice were rested (10 weeks) and challenged with pRBCs or GPI-KLH to assess memory B cell recall against foreign antigen. We found that GPI-specific B cells were detectable in GPI-KLH vaccinated mice, but not in Plasmodium-infected mice. Additionally, in previously vaccinated mice GPI-specific IgG1 MBCs were reactivated against both pRBCs and synthetic GPI-KLH, which resulted in increased serum levels of anti-GPI IgG in both challenge approaches. Collectively our findings contribute to the understanding of B cell immunity in malaria and have important clinical implications for inclusion of carbohydrate conjugates in malaria vaccines.
- Publisher
- Frontiers Media
- Research Division(s)
- Population Health And Immunity
- PubMed ID
- 31447848
- Publisher's Version
- https://doi.org/10.3389/fimmu.2019.01840
- Open Access at Publisher's Site
https://doi.org/10.3389/fimmu.2019.01840- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2019-09-20 10:16:32
Last Modified: 2019-09-24 03:47:54