Cytokine release and gastrointestinal symptoms after gluten challenge in celiac disease
- Author(s)
- Goel, G; Tye-Din, JA; Qiao, SW; Russell, AK; Mayassi, T; Ciszewski, C; Sarna, VK; Wang, S; Goldstein, KE; Dzuris, JL; Williams, LJ; Xavier, RJ; Lundin, KEA; Jabri, B; Sollid, LM; Anderson, RP;
- Details
- Publication Year 2019-08,Volume 5,Issue #8,Page eaaw7756
- Journal Title
- Scientific Advances
- Publication Type
- Journal Article
- Abstract
- Celiac disease (CeD), caused by immune reactions to cereal gluten, is treated with gluten -elimination diets. Within hours of gluten exposure, either perorally or extraorally by intradermal injection, treated patients experience gastrointestinal symptoms. To test whether gluten exposure leads to systemic cytokine production time -related to symptoms, series of multiplex cytokine measurements were obtained in CeD patients after gluten challenge. Peptide injection elevated at least 15 plasma cytokines, with IL-2, IL-8, and IL-10 being most prominent (fold-change increase at 4 hours of 272, 11, and 1.2, respectively). IL-2 and IL-8 were the only cytokines elevated at 2 hours, preceding onset of symptoms. After gluten ingestion, IL-2 was the earliest and most prominent cytokine (15-fold change at 4 hours). Supported by studies of patient-derived gluten-specific T cell clones and primary lymphocytes, our observations indicate that gluten-specific CD4(+) T cells are rapidly reactivated by antigen -exposure likely causing CeD-associated gastrointestinal symptoms.
- Publisher
- AAAS
- Research Division(s)
- Immunology
- PubMed ID
- 31457091
- Publisher's Version
- https://doi.org/10.1126/sciadv.aaw7756
- Open Access at Publisher's Site
https://doi.org/10.1126/sciadv.aaw7756- Terms of Use/Rights Notice
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Creation Date: 2019-09-20 10:16:31
Last Modified: 2020-04-02 02:22:11