Viral MLKL homologs subvert necroptotic cell death by sequestering cellular RIPK3
Details
Publication Year 2019-09-24, Volume 28, Issue #13, Page 3309-3319 e5
Journal Title
Cell Reports
Publication Type
Journal Article
Abstract
Necroptotic cell death has been implicated in many human pathologies and is thought to have evolved as an innate immunity mechanism. The pathway relies on two key effectors: the kinase receptor-interacting protein kinase 3 (RIPK3) and the terminal effector, the pseudokinase mixed-lineage kinase-domain-like (MLKL). We identify proteins with high sequence similarity to the pseudokinase domain of MLKL in poxvirus genomes. Expression of these proteins from the BeAn 58058 and Cotia poxviruses, but not swinepox, in human and mouse cells blocks cellular MLKL activation and necroptotic cell death. We show that viral MLKL-like proteins function as dominant-negative mimics of host MLKL, which inhibit necroptosis by sequestering RIPK3 via its kinase domain to thwart MLKL engagement and phosphorylation. These data support an ancestral role for necroptosis in defense against pathogens. Furthermore, mimicry of a cellular pseudokinase by a pathogen adds to the growing repertoire of functions performed by pseudokinases in signal transduction.
Publisher
Elsevier
WEHI Research Division(s)
Inflammation; Advanced Technology And Biology; Chemical Biology
PubMed ID
31553902
Open Access at Publisher's Site
https://doi.org/110.1016/j.celrep.2019.08.055
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2019-10-23 02:22:54
Last Modified: 2019-10-23 02:45:43
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