Class-switch recombination occurs infrequently in germinal centers
- Author(s)
- Roco, JA; Mesin, L; Binder, SC; Nefzger, C; Gonzalez-Figueroa, P; Canete, PF; Ellyard, J; Shen, Q; Robert, PA; Cappello, J; Vohra, H; Zhang, Y; Nowosad, CR; Schiepers, A; Corcoran, LM; Toellner, KM; Polo, JM; Meyer-Hermann, M; Victora, G; Vinuesa, CG;
- Details
- Publication Year 2019-07-26,Volume 51,Issue #2,Page 237-350.e7
- Journal Title
- Immunity
- Publication Type
- Journal Article
- Abstract
- Class-switch recombination (CSR) is a DNA recombination process that replaces the immunoglobulin (Ig) constant region for the isotype that can best protect against the pathogen. Dysregulation of CSR can cause self-reactive BCRs and B cell lymphomas; understanding the timing and location of CSR is therefore important. Although CSR commences upon T cell priming, it is generally considered a hallmark of germinal centers (GCs). Here, we have used multiple approaches to show that CSR is triggered prior to differentiation into GC B cells or plasmablasts and is greatly diminished in GCs. Despite finding a small percentage of GC B cells expressing germline transcripts, phylogenetic trees of GC BCRs from secondary lymphoid organs revealed that the vast majority of CSR events occurred prior to the onset of somatic hypermutation. As such, we have demonstrated the existence of IgM-dominated GCs, which are unlikely to occur under the assumption of ongoing switching.
- Publisher
- Cell Press
- Research Division(s)
- Immunology
- PubMed ID
- 31375460
- Publisher's Version
- https://doi.org/10.1016/j.immuni.2019.07.001
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2019-08-13 12:35:08
Last Modified: 2019-08-23 02:25:54