Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder
Journal Title
Stem Cell Research
Publication Type
Journal Article
Abstract
We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PBMCs was performed using non-integrative Sendai virus containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype and pluripotency was validated by immunofluorescence, flow cytometry and their ability to differentiate into the three embryonic germ layers. These iPSC lines are a valuable resource to study the molecular mechanisms underlying ASD.
Publisher
Elsevier
Research Division(s)
Population Health And Immunity
PubMed ID
31415975
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2019-08-21 11:19:28
Last Modified: 2019-08-21 11:20:49
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