Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder
- Author(s)
- Bozaoglu, K; Gao, Y; Stanley, E; Fanjul-Fernandez, M; Brown, NJ; Pope, K; Green, CC; Vlahos, K; Sourris, K; Bahlo, M; Delatycki, M; Scheffer, I; Lockhart, PJ;
- Journal Title
- Stem Cell Research
- Publication Type
- Journal Article
- Abstract
- We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PBMCs was performed using non-integrative Sendai virus containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype and pluripotency was validated by immunofluorescence, flow cytometry and their ability to differentiate into the three embryonic germ layers. These iPSC lines are a valuable resource to study the molecular mechanisms underlying ASD.
- Publisher
- Elsevier
- Research Division(s)
- Population Health And Immunity
- PubMed ID
- 31415975
- Publisher's Version
- https://doi.org/10.1016/j.scr.2019.101516
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2019-08-21 11:19:28
Last Modified: 2019-08-21 11:20:49