Hhex regulates murine lymphoid progenitor survival independently of Stat5 and Cdkn2a
Journal Title
European Journal of Immunology
Publication Type
Journal epub ahead of print
Abstract
The transcription factor Hhex (Hematopoietically-expressed homeobox gene) is critical for development of multiple lymphoid lineages beyond the Common Lymphoid Progenitor (CLP). In addition, Hhex regulates hematopoietic stem cell (HSC) self-renewal, emergency hematopoiesis and acute myeloid leukemia (AML) initiation and maintenance. Hhex mediates its effects on HSCs and AML stem cells via repression of the Cdkn2a tumor suppressor locus. However, we report here that loss of Cdkn2a does not rescue the failure of lymphoid development caused by loss of Hhex. As loss of Hhex causes apoptosis of lymphoid progenitors associated with impaired Bcl2 expression and defective Stat5b signaling, we tested the effects of rescuing these pathways using transgenic mice. Expression of the anti-apoptotic factor Bcl2, but not activated Stat5, rescued the development of T-, B- and Natural Killer cell lineages in the absence of Hhex. These results indicate that Bcl2 expression, but not Stat5b signaling or loss of Cdkn2a, can overcome the lymphoid deficiencies caused by the absence of Hhex, suggesting that the primary role of this transcription factor is to promote survival of lymphoid progenitors during early lymphoid development. This article is protected by copyright. All rights reserved.
Publisher
Wiley
WEHI Research Division(s)
Immunology
PubMed ID
32090320
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2020-05-04 11:46:55
Last Modified: 2020-05-04 02:20:25
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