Single-cell lineage tracing approaches in hematology research - technical considerations
Journal Title
Experimental Hematology
Publication Type
Journal epub ahead of print
Abstract
The coordinated differentiation of hematopoietic stem and progenitor cells (HSPCs) into the various mature blood cell types is responsible for sustaining blood and immune system homeostasis. The cell fate decisions underlying this important biological process are made at the level of single cells. Methods to trace the fate of single cells are therefore essential for understanding the hematopoietic system activity in health and disease, and have made a major impact in how we understand and represent hematopoiesis. Here, we discuss the basic methodologies and technical considerations for three important clonal assays: single cell transplantation, lentiviral barcoding, and sleeping beauty barcoding. This Perspective is a synthesis of presentations and discussions from the 2019 International Society for Experimental Hematology (ISEH) Annual Meeting New Investigator Technology Session and the 2019 ISEH Winter Webinar.
Publisher
Elsevier
WEHI Research Division(s)
Immunology
PubMed ID
32735908
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2020-08-05 11:11:22
Last Modified: 2020-08-05 11:38:48
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