Leukocyte Tetraspanin CD53 Restrains alpha3 Integrin Mobilization and Facilitates Cytoskeletal Remodeling and Transmigration in Mice
Journal Title
J Immunol
Publication Type
Journal epub ahead of print
Abstract
The importance of tetraspanin proteins in regulating migration has been demonstrated in many diverse cellular systems. However, the function of the leukocyte-restricted tetraspanin CD53 remains obscure. We therefore hypothesized that CD53 plays a role in regulating leukocyte recruitment and tested this hypothesis by examining responses of CD53-deficient mice to a range of inflammatory stimuli. Deletion of CD53 significantly reduced neutrophil recruitment to the acutely inflamed peritoneal cavity. Intravital microscopy revealed that in response to several inflammatory and chemotactic stimuli, absence of CD53 had only minor effects on leukocyte rolling and adhesion in postcapillary venules. In contrast, Cd53(-/-) mice showed a defect in leukocyte transmigration induced by TNF, CXCL1 and CCL2, and a reduced capacity for leukocyte retention on the endothelial surface under shear flow. Comparison of adhesion molecule expression in wild-type and Cd53(-/-) neutrophils revealed no alteration in expression of beta2 integrins, whereas L-selectin was almost completely absent from Cd53(-/-) neutrophils. In addition, Cd53(-/-) neutrophils showed defects in activation-induced cytoskeletal remodeling and translocation to the cell periphery, responses necessary for efficient transendothelial migration, as well as increased alpha3 integrin expression. These alterations were associated with effects on inflammation, so that in Cd53(-/-) mice, the onset of neutrophil-dependent serum-induced arthritis was delayed. Together, these findings demonstrate a role for tetraspanin CD53 in promotion of neutrophil transendothelial migration and inflammation, associated with CD53-mediated regulation of L-selectin expression, attachment to the endothelial surface, integrin expression and trafficking, and cytoskeletal function.
Publisher
ASI
Research Division(s)
Inflammation
PubMed ID
32532837
NHMRC Grants
NHMRC/1113577
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2020-06-15 10:33:58
Last Modified: 2020-06-15 10:43:37
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