A missense mutation in the MLKL brace region promotes lethal neonatal inflammation and hematopoietic dysfunction
Details
Publication Year 2020-06-19, Volume 11, Issue #1, Page 3150
Journal Title
Nature Communications
Publication Type
Journal Article
Abstract
MLKL is the essential effector of necroptosis, a form of programmed lytic cell death. We have isolated a mouse strain with a single missense mutation, Mlkl(D139V), that alters the two-helix 'brace' that connects the killer four-helix bundle and regulatory pseudokinase domains. This confers constitutive, RIPK3 independent killing activity to MLKL. Homozygous mutant mice develop lethal postnatal inflammation of the salivary glands and mediastinum. The normal embryonic development of Mlkl(D139V) homozygotes until birth, and the absence of any overt phenotype in heterozygotes provides important in vivo precedent for the capacity of cells to clear activated MLKL. These observations offer an important insight into the potential disease-modulating roles of three common human MLKL polymorphisms that encode amino acid substitutions within or adjacent to the brace region. Compound heterozygosity of these variants is found at up to 12-fold the expected frequency in patients that suffer from a pediatric autoinflammatory disease, chronic recurrent multifocal osteomyelitis (CRMO).
Publisher
NPG
WEHI Research Division(s)
Inflammation; Blood Cells And Blood Cancer; Infectious Diseases And Immune Defence; Population Health And Immunity; Structural Biology
PubMed ID
32561755
Open Access at Publisher's Site
https://doi.org/10.1038/s41467-020-16819-z
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2020-06-22 11:52:54
Last Modified: 2020-06-22 12:09:40
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