NOD1 is required for Helicobacter pylori induction of IL-33 responses in gastric epithelial cells
Journal Title
Cell Microbiol
Publication Type
Journal Article in press
Abstract
Helicobacter pylori (H. pylori) causes chronic inflammation which is a key precursor to gastric carcinogenesis. It has been suggested that H. pylori may limit this immunopathology by inducing the production of interleukin 33 (IL-33) in gastric epithelial cells, thus promoting T helper 2 immune responses. The molecular mechanism underlying IL-33 production in response to H. pylori infection, however, remains unknown. In this study, we demonstrate that H. pylori activates signalling via the pathogen recognition molecule Nucleotide-Binding Oligomerisation Domain-Containing Protein 1 (NOD1) and its adaptor protein receptor-interacting serine-threonine Kinase 2, to promote production of both full-length and processed IL-33 in gastric epithelial cells. Furthermore, IL-33 responses were dependent on the actions of the H. pylori Type IV secretion system, required for activation of the NOD1 pathway, as well as on the Type IV secretion system effector protein, CagA. Importantly, Nod1(+/+) mice with chronic H. pylori infection exhibited significantly increased gastric IL-33 and splenic IL-13 responses, but decreased IFN-gamma responses, when compared with Nod1(-/-) animals. Collectively, our data identify NOD1 as an important regulator of mucosal IL-33 responses in H. pylori infection. We suggest that NOD1 may play a role in protection against excessive inflammation.
Publisher
Wiley
Research Division(s)
Cell Signalling And Cell Death
PubMed ID
29392836
NHMRC Grants
NHMRC/1079930NHMRC/1079904
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2018-02-28 08:05:08
Last Modified: 2018-02-28 08:56:52
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