RLE plots: Visualizing unwanted variation in high dimensional data
- Author(s)
- Gandolfo, LC; Speed, TP;
- Details
- Publication Year 2018,Volume 13,Issue #2,Page e0191629
- Journal Title
- PLoS One
- Publication Type
- Journal Article
- Abstract
- Unwanted variation can be highly problematic and so its detection is often crucial. Relative log expression (RLE) plots are a powerful tool for visualizing such variation in high dimensional data. We provide a detailed examination of these plots, with the aid of examples and simulation, explaining what they are and what they can reveal. RLE plots are particularly useful for assessing whether a procedure aimed at removing unwanted variation, i.e. a normalization procedure, has been successful. These plots, while originally devised for gene expression data from microarrays, can also be used to reveal unwanted variation in many other kinds of high dimensional data, where such variation can be problematic.
- Publisher
- PLOS
- Research Division(s)
- Bioinformatics
- PubMed ID
- 29401521
- Publisher's Version
- https://doi.org/10.1371/journal.pone.0191629
- Open Access at Publisher's Site
https://doi.org/10.1371/journal.pone.0191629- NHMRC Grants
- NHMRC/1054618,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2018-02-28 08:04:55
Last Modified: 2018-02-28 08:15:22