Combining cell type-restricted adenoviral targeting with immunostaining and flow cytometry to identify cells-of-origin of lung cancer
- Author(s)
- Best, SA; Kersbergen, A; Asselin-Labat, ML; Sutherland, KD;
- Journal Title
- Inflammation and Cancer. Methods in Molecular Biology, vol 1725
- Abstract
- Lung cancers display considerable intertumoral heterogeneity, leading to the classification of distinct tumor subtypes. Our understanding of the genetic aberrations that underlie tumor subtypes has been greatly enhanced by recent genomic sequencing studies and state-of-the-art gene targeting technologies, highlighting evidence that distinct lung cancer subtypes may be derived from different "cells-of-origin". Here, we describe the intra-tracheal delivery of cell type-restricted Ad5-Cre viruses into the lungs of adult mice, combined with immunohistochemical and flow cytometry strategies for the detection of lung cancer-initiating cells in vivo.
- Publisher
- Humana Press
- Research Division(s)
- Stem Cells And Cancer
- PubMed ID
- 29322405
- Publisher's Version
- https://doi.org/10.1007/978-1-4939-7568-6_2
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2018-02-27 12:34:54
Last Modified: 2018-02-27 01:07:19