Transcriptional profiling of eosinophil subsets in interleukin-5 transgenic mice
- Author(s)
- Fairfax, KA; Bolden, JE; Robinson, AJ; Lucas, EC; Baldwin, TM; Ramsay, KA; Cole, R; Hilton, DJ; de Graaf, CA;
- Details
- Publication Year 2018-05-14,Volume 104,Issue #1,Page 195-204
- Journal Title
- Journal of Leukocyte Biology
- Publication Type
- Journal Article
- Abstract
- Eosinophils are important in fighting parasitic infections and are implicated in the pathogenesis of asthma and allergy. IL-5 is a critical regulator of eosinophil development, controlling proliferation, differentiation, and maturation of the lineage. Mice that constitutively express IL-5 have in excess of 10-fold more eosinophils in the hematopoietic organs than their wild type (WT) counterparts. We have identified that much of this expansion is in a population of Siglec-F high eosinophils, which are rare in WT mice. In this study, we assessed transcription in myeloid progenitors, eosinophil precursors, and Siglec-F medium and Siglec-F high eosinophils from IL-5 transgenic mice and in doing so have created a useful resource for eosinophil biologists. We have then utilized these populations to construct an eosinophil trajectory based on gene expression and to identify gene sets that are associated with eosinophil lineage progression. Cell cycle genes were significantly associated with the trajectory, and we experimentally demonstrate an increasing trend toward quiescence along the trajectory. Additionally, we found gene expression changes associated with constitutive IL-5 signaling in eosinophil progenitors, many of which were not observed in eosinophils.
- Publisher
- Wiley
- Research Division(s)
- Molecular Medicine
- PubMed ID
- 29758105
- Publisher's Version
- https://doi.org/10.1002/JLB.6MA1117-451R
- Open Access at Publisher's Site
https://doi.org/10.1002/JLB.6MA1117-451R- NHMRC Grants
- NHMRC/637403, NHMRC/1035229,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2018-05-18 09:33:24
Last Modified: 2018-10-22 11:45:40