Analysis of histone modifications in acute myeloid leukaemia using chromatin immunoprecipitation
Journal Title
Inflammation and Cancer. Methods in Molecular Biology, vol 1725
Publication Type
Book Section
Abstract
Chromatin Immunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analysis (ChIP-Seq). Here we describe the procedure for ChIP of histone marks in myeloid leukaemia cell lines and the subsequent purification of genomic DNA associated with repressive and activating histone modifications for further analysis. This procedure can be widely applied to a variety of histone marks to assess both activating and repressive modifications in the context of myeloid leukaemia.
Publisher
Humana Press
WEHI Research Division(s)
Molecular Medicine
PubMed ID
29322418
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2018-03-27 09:20:17
Last Modified: 2018-03-27 09:33:53
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