Analysis of histone modifications in acute myeloid leukaemia using chromatin immunoprecipitation
- Author(s)
- Shields, BJ; Keniry, A; Blewitt, ME; McCormack, MP;
- Journal Title
- Inflammation and Cancer. Methods in Molecular Biology, vol 1725
- Publication Type
- Book Section
- Abstract
- Chromatin Immunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analysis (ChIP-Seq). Here we describe the procedure for ChIP of histone marks in myeloid leukaemia cell lines and the subsequent purification of genomic DNA associated with repressive and activating histone modifications for further analysis. This procedure can be widely applied to a variety of histone marks to assess both activating and repressive modifications in the context of myeloid leukaemia.
- Publisher
- Humana Press
- Research Division(s)
- Molecular Medicine
- PubMed ID
- 29322418
- Publisher's Version
- https://doi.org/10.1007/978-1-4939-7568-6_15
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2018-03-27 09:20:17
Last Modified: 2018-03-27 09:33:53