Expression and purification of JAK1 and SOCS1 for structural and biochemical studies
- Journal Title
- Inflammation and Cancer. Methods in Molecular Biology, vol 1725
- Publication Type
- Book Section
- Abstract
- Interferon gamma (IFNgamma) is a potent inflammatory and immune cytokine. IFNgamma signals via the interferon gamma receptor (IFNGR), which is constitutively bound to Janus Kinase (JAK) 1 and JAK2 via its intracellular domain. These two JAK proteins then initiate the inflammatory signaling cascade. The most potent inhibitor of IFNgamma signaling is Suppressor of Cytokine Signaling 1 (SOCS1). SOCS1 negatively regulates IFNgamma signaling pathway (and other pathways) by directly inhibiting JAKs. Here, we describe a protocol for the recombinant production and purification of the JAK1 kinase domain and its inhibitor SOCS1, for structural and biochemical studies.
- Publisher
- Humana Press
- Research Division(s)
- Structural Biology
- PubMed ID
- 29322424
- Publisher's Version
- https://doi.org/10.1007/978-1-4939-7568-6_21
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2018-03-27 09:20:15
Last Modified: 2018-03-27 09:22:11