TERT structural rearrangements in metastatic pheochromocytomas
Journal Title
Endocrine Related Cancer
Publication Type
Journal Article in press
Abstract
Pheochromocytomas (PC) and paragangliomas (PGL) are endocrine tumors for which the genetic and clinico-pathological features of metastatic progression remain incompletely understood. As a result, the risk of metastasis from a primary tumor cannot be predicted. Early diagnosis of individuals at high risk of developing metastases is clinically important and the identification of new biomarkers that are predictive of metastatic potential is of high value. Activation of TERT has been associated with a number of malignant tumors, including PC/PGL. However, the mechanism of TERT activation in the majority of PC/PGL remains unclear. As TERT promoter mutations occur rarely in PC/PGL, we hypothesized that other mechanisms - such as structural variations - may underlie TERT activation in these tumors. From 35 PC and four PGL, we identified three primary PC that developed metastases with elevated TERT expression, each of which lacked TERT promoter mutations and promoter DNA methylation. Using whole genome sequencing we identified somatic structural alterations proximal to the TERT locus in two of these tumors. In both tumors, the genomic rearrangements led to positioning of super-enhancers proximal to the TERT promoter, that are likely responsible for activation of the normally tightly repressed TERT expression in chromaffin cells.
Publisher
Bioscientifica
Research Division(s)
Bioinformatics
PubMed ID
28974544
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2017-10-16 01:59:54
Last Modified: 2017-10-16 02:37:07
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