Dynein light chain regulates adaptive and innate B cell development by distinctive genetic mechanisms
- Author(s)
- KING, A; Li, L; Wong, DM; Liu, R; Bamford, R; Strasser, A; Tarlinton, DM; Heierhorst, J;
- Details
- Publication Year 2017-09,Volume 13,Issue #9,Page e1007010
- Journal Title
- PLoS Genetics
- Publication Type
- Journal Article
- Abstract
- Mechanistic differences in the development and function of adaptive, high-affinity antibody-producing B-2 cells and innate-like, "natural" antibody-producing B-1a cells remain poorly understood. Here we show that the multi-functional dynein light chain (DYNLL1/LC8) plays important roles in the establishment of B-1a cells in the peritoneal cavity and in the ongoing development of B-2 lymphoid cells in the bone marrow of mice. Epistasis analyses indicate that Dynll1 regulates B-1a and early B-2 cell development in a single, linear pathway with its direct transcriptional activator ASCIZ (ATMIN/ZNF822), and that the two genes also have complementary functions during late B-2 cell development. The B-2 cell defects caused by loss of DYNLL1 were associated with lower levels of the anti-apoptotic protein BCL-2, and could be supressed by deletion of pro-apoptotic BIM which is negatively regulated by both DYNLL1 and BCL-2. Defects in B cell development caused by loss of DYNLL1 could also be partially suppressed by a pre-arranged SWHEL Igm-B cell receptor transgene. In contrast to the rescue of B-2 cell numbers, the B-1a cell deficiency in Dynll1-deleted mice could not be suppressed by the loss of Bim, and was further compounded by the SWHEL transgene. Conversely, oncogenic MYC expression, which is synthetic lethal with Dynll1 deletion in B-2 cells, did not further reduce B-1a cell numbers in Dynll1-defcient mice. Finally, we found that the ASCIZ-DYNLL1 axis was also required for the early-juvenile development of aggressive MYC-driven and p53-deficient B cell lymphomas. These results identify ASCIZ and DYNLL1 as the core of a transcriptional circuit that differentially regulates the development of the B-1a and B-2 B lymphoid cell lineages and plays a critical role in lymphomagenesis.
- Publisher
- PLOS
- Research Division(s)
- Molecular Genetics Of Cancer
- PubMed ID
- 28922373
- Publisher's Version
- https://doi.org/10.1371/journal.pgen.1007010
- Open Access at Publisher's Site
http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007010- NHMRC Grants
- NHMRC/1020363, NHMRC/1016701, NHMRC/1054925, NHMRC/1060675,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2017-10-16 02:00:02
Last Modified: 2017-10-17 08:06:41