Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

Liu, X; Nefzger, CM; Rossello, FJ; Chen, J; Knaupp, AS; Firas, J; Ford, E; Pflueger, J; Paynter, JM; Chy, HS; O&#39;Brien, CM; Huang, C; Mishra, K; Hodgson-Garms, M; Jansz, N; Williams, SM; Blewitt, ME; Nilsson, SK; Schittenhelm, RB; Laslett, AL; Lister, R; Polo, JM

Author(s): Liu, X; Nefzger, CM; Rossello, FJ; Chen, J; Knaupp, AS; Firas, J; Ford, E; Pflueger, J; Paynter, JM; Chy, HS; O'Brien, CM; Huang, C; Mishra, K; Hodgson-Garms, M; Jansz, N; Williams, SM; Blewitt, ME; Nilsson, SK; Schittenhelm, RB; Laslett, AL; Lister, R; Polo, JM;
Details: Publication Year 2017-11,Volume 14,Issue #11,Page 1055-1062
Journal Title: Nature Methods
Publication Type: Journal Article
Abstract: Recent reports on the characteristics of naive human pluripotent stem cells (hPSCs) obtained using independent methods differ. Naive hPSCs have been mainly derived by conversion from primed hPSCs or by direct derivation from human embryos rather than by somatic cell reprogramming. To provide an unbiased molecular and functional reference, we derived genetically matched naive hPSCs by direct reprogramming of fibroblasts and by primed-to-naive conversion using different naive conditions (NHSM, RSeT, 5iLAF and t2iLGoY). Our results show that hPSCs obtained in these different conditions display a spectrum of naive characteristics. Furthermore, our characterization identifies KLF4 as sufficient for conversion of primed hPSCs into naive t2iLGoY hPSCs, underscoring the role that reprogramming factors can play for the derivation of bona fide naive hPSCs.
Publisher: Springer Nature
Research Division(s): Molecular Medicine
PubMed ID: 28945704
Publisher's Version: https://doi.org/10.1038/nmeth.4436
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2017-10-16 02:00:06

Last Modified: 2017-11-29 08:35:58