Chemokine receptor-dependent control of skin tissue-resident memory T cell formation
Details
Publication Year 2017-10-01,Volume 199,Issue #7,Page 2451-2459
Journal Title
Journal of Immunology
Publication Type
Journal Article
Abstract
Infection or inflammation of the skin recruits effector CD8+ T cells that enter the epidermis and form populations of long-lived tissue-resident memory T (TRM) cells. These skin TRM cells migrate within the constrained epidermal environment by extending multiple dynamic dendritic projections and squeezing between keratinocytes to survey the tissue for pathogens. In this study, we examined the signals required for this distinctive mode of T cell migration by inhibiting key cytoskeletal components and performing intravital two-photon microscopy to visualize TRM cell behavior. We found that TRM cell motility and dendrite formation required an intact actomyosin cytoskeleton and the Rho-associated coiled-coil containing kinases. We also identified an essential role for microtubules for maintaining skin TRM cell shape and cellular integrity. We reveal a role for pertussis toxin-sensitive signaling for TRM cell dendritic morphology and migration that is independent of CXCR3 or CXCR6, or the skin-selective chemokine receptors CCR10 and CCR8. However, we found that CXCR6 and CCR10 expression by CD8+ T cells was required for the optimal formation of memory T cell populations, in particular TRM cell populations in the skin.
Publisher
ASI
Research Division(s)
Molecular Immunology
PubMed ID
28855310
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2017-09-06 11:18:10
Last Modified: 2017-11-29 08:43:07
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