Generation of genetic knockouts in myeloid cell lines using a lentiviral CRISPR/Cas9 system
- Author(s)
- Baker, PJ; Masters, SL;
- Journal Title
- Methods Mol Biol
- Publication Type
- Journal Article
- Abstract
- CRISPR/Cas9-based gene targeting allows deletion of a gene of interest from cultured cell lines. Due to difficulty in transiently transfecting hematopoetic cells with components required for this process, we have adopted a lentiviral system for delivery of the CRISPR/Cas9 components into myeloid cell lines. Here, we detail the process of knocking out genes from pools of cultured myeloid cells using this CRISPR/Cas9 system and describe methods of validating these knockout pools.
- Publisher
- Springer
- Research Division(s)
- Inflammation
- PubMed ID
- 29177854
- Publisher's Version
- https://doi.org/10.1007/978-1-4939-7519-8_3
- NHMRC Grants
- NHMRC/1057815, NHMRC/1099262,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2018-01-15 09:30:07
Last Modified: 2021-03-29 08:21:07