Generation of genetic knockouts in myeloid cell lines using a lentiviral CRISPR/Cas9 system
Author(s)
Baker, PJ; Masters, SL;
Journal Title
Methods Mol Biol
Publication Type
Journal Article
Abstract
CRISPR/Cas9-based gene targeting allows deletion of a gene of interest from cultured cell lines. Due to difficulty in transiently transfecting hematopoetic cells with components required for this process, we have adopted a lentiviral system for delivery of the CRISPR/Cas9 components into myeloid cell lines. Here, we detail the process of knocking out genes from pools of cultured myeloid cells using this CRISPR/Cas9 system and describe methods of validating these knockout pools.
Publisher
Springer
WEHI Research Division(s)
Inflammation
PubMed ID
29177854
NHMRC Grants
NHMRC/1057815 NHMRC/1099262
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2018-01-15 09:30:07
Last Modified: 2021-03-29 08:21:07
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