Generation of innate immune reporter cells using retroviral transduction
- Author(s)
- Gloria, YC; Latz, E; De Nardo, D;
- Journal Title
- Methods Mol Biol
- Publication Type
- Journal Article
- Abstract
- Innate immune cells are notoriously difficult to transfect; however, retroviruses can be used to stably integrate genes of interest into the host genome of primary or immortalized immune cells resulting in the generation of reporter cells. Here, we provide a detailed protocol covering the production of retroviruses, retroviral infection of innate immune target cells (including isolation and differentiation of murine bone marrow cells to macrophages), and several methods for enrichment of positively transduced cells.
- Publisher
- Humana Press Inc.
- Research Division(s)
- Inflammation
- PubMed ID
- 29177858
- Publisher's Version
- https://doi.org/10.1007/978-1-4939-7519-8_7
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2018-01-15 09:30:08
Last Modified: 2018-02-14 03:51:50