Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling

Pal, B; Chen, Y; Vaillant, F; Jamieson, P; Gordon, L; Rios, AC; Wilcox, S; Fu, N; Liu, KH; Jackling, FC; Davis, MJ; Lindeman, GJ; Smyth, GK; Visvader, JE

Author(s): Pal, B; Chen, Y; Vaillant, F; Jamieson, P; Gordon, L; Rios, AC; Wilcox, S; Fu, N; Liu, KH; Jackling, FC; Davis, MJ; Lindeman, GJ; Smyth, GK; Visvader, JE;
Details: Publication Year 2017-11-20,Volume 8,Issue #1,Page 1627
Journal Title: Nat Commun
Publication Type: Journal Article
Abstract: The mammary epithelium comprises two primary cellular lineages, but the degree of heterogeneity within these compartments and their lineage relationships during development remain an open question. Here we report single-cell RNA profiling of mouse mammary epithelial cells spanning four developmental stages in the post-natal gland. Notably, the epithelium undergoes a large-scale shift in gene expression from a relatively homogeneous basal-like program in pre-puberty to distinct lineage-restricted programs in puberty. Interrogation of single-cell transcriptomes reveals different levels of diversity within the luminal and basal compartments, and identifies an early progenitor subset marked by CD55. Moreover, we uncover a luminal transit population and a rare mixed-lineage cluster amongst basal cells in the adult mammary gland. Together these findings point to a developmental hierarchy in which a basal-like gene expression program prevails in the early post-natal gland prior to the specification of distinct lineage signatures, and the presence of cellular intermediates that may serve as transit or lineage-primed cells.
Publisher: Springer Nature
Research Division(s): Bioinformatics; Stem Cells And Cancer; Systems Biology And Personalised Medicine
PubMed ID: 29158510
Publisher's Version: https://doi.org/10.1038/s41467-017-01560-x
NHMRC Grants: NHMRC/1016701, NHMRC/1054618, NHMRC/1100807, NHMRC/1113133, NHMRC/1078730, NHMRC/1058892, NHMRC/1037230, NHMRC/1102742,
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Creation Date: 2018-01-15 09:30:20

Last Modified: 2018-01-15 10:11:04