Synthesis and use of 6,6,6-trifluoro-L-fucose to block core-fucosylation in hybridoma cell lines
- Author(s)
- McKenzie, NC; Scott, NE; John, A; White, JM; Goddard-Borger, ED;
- Journal Title
- Carbohydrate Research
- Publication Type
- Journal Article
- Abstract
- Many monoclonal antibodies (mAbs) used in cancer immunotherapy mediate tumour cell lysis by recruiting natural killer (NK) cells; a phenomenon known as antibody-dependent cellular cytotoxicity (ADCC). Eliminating core-fucose from the N-glycans of a mAb enhances its capacity to induce ADCC. As such, inhibitors of fucosylation are highly desirable for the production of mAbs for research and therapeutic use. Herein, we describe a simple synthesis of 6,6,6-trifluoro-l-fucose (F3Fuc), a metabolic inhibitor of fucosylation, and demonstrate the utility of this molecule in the production of low-fucose mAbs from murine hybridoma cell lines.
- Publisher
- Elsevier
- Research Division(s)
- Chemical Biology
- PubMed ID
- 29874559
- Publisher's Version
- https://doi.org/10.1016/j.carres.2018.05.008
- NHMRC Grants
- NHMRC/1100164, NHMRC/1037373,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2018-06-26 12:34:44
Last Modified: 2019-08-05 11:54:25