Assessing the role of the T-box transcription factor Eomes in B cell differentiation during either Th1 or Th2 cell-biased responses
Details
Publication Year 2018, Volume 13, Issue #12, Page e0208343
Journal Title
PLoS One
Publication Type
Journal Article
Abstract
Successful T-dependent humoral responses require the production of antibody-secreting plasmablasts, as well as the formation of germinal centers which eventually form high-affinity B cell memory. The ability of B cells to differentiate into germinal center and plasma cells, as well as the ability to tailor responses to different pathogens, is driven by transcription factors. In T cells, the T-box transcription factors T-bet and Eomesodermin (Eomes) regulate effector and memory T cell differentiation, respectively. While T-bet has a critical role in regulating anti-viral B cell responses, a role for Eomes in B cells has yet to be described. We therefore investigated whether Eomes was required for B cell differentiation during either Th1 or Th2 cell-biased immune responses. Here, we demonstrate that deletion of Eomes specifically in B cells did not affect B cell differentiation in response to vaccination, as well as following viral or helminth infection. In contrast to its established role in CD8+ T cells, Eomes did not influence memory B cell differentiation. Finally, the use of an Eomes reporter mouse confirmed the lack of Eomes expression during immune responses. Thus, germinal center and plasma cell differentiation and the formation of isotype-switched memory B cells in response to infection are independent of Eomes expression.
Publisher
PLOS
WEHI Research Division(s)
Molecular Immunology
PubMed ID
30521606
Open Access at Publisher's Site
https://doi.org/10.1371/journal.pone.0208343
NHMRC Grants
NHMRC/1054925 NHMRC/1135898
ARC Grants
ARC/FT130100708,
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2018-12-18 02:54:28
Last Modified: 2018-12-18 03:11:57
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