Smchd1 Targeting to the Inactive X Is dependent on the Xist-HnrnpK-PRC1 pathway
Details
Publication Year 2018-11-13, Volume 25, Issue #7, Page 1912-1923 e9
Journal Title
Cell Reports
Publication Type
Journal Article
Abstract
We and others have recently reported that the SMC protein Smchd1 is a regulator of chromosome conformation. Smchd1 is critical for the structure of the inactive X chromosome and at autosomal targets such as the Hox genes. However, it is unknown how Smchd1 is recruited to these sites. Here, we report that Smchd1 localizes to the inactive X via the Xist-HnrnpK-PRC1 (polycomb repressive complex 1) pathway. Contrary to previous reports, Smchd1 does not bind Xist or other RNA molecules with any specificity. Rather, the localization of Smchd1 to the inactive X is H2AK119ub dependent. Following perturbation of this interaction, Smchd1 is destabilized, which has consequences for gene silencing genome-wide. Our work adds Smchd1 to the PRC1 silencing pathway for X chromosome inactivation.
Publisher
Elsevier
WEHI Research Division(s)
Molecular Medicine; Systems Biology And Personalised Medicine; Cell Signalling And Cell Death
PubMed ID
30428357
Open Access at Publisher's Site
https://doi.org/10.1016/j.celrep.2018.10.044
NHMRC Grants
NHMRC/1098290 NHMRC/1105754
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2018-11-20 03:13:38
Last Modified: 2018-11-20 03:14:54
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