A FRET biosensor for necroptosis uncovers two different modes of the release of DAMPs
- Author(s)
- Murai, S; Yamaguchi, Y; Shirasaki, Y; Yamagishi, M; Shindo, R; Hildebrand, JM; Miura, R; Nakabayashi, O; Totsuka, M; Tomida, T; Adachi-Akahane, S; Uemura, S; Silke, J; Yagita, H; Miura, M; Nakano, H;
- Details
- Publication Year 2018-10-26,Volume 9,Issue #1,Page 4457
- Journal Title
- Nature Communications
- Publication Type
- Journal Article
- Abstract
- Necroptosis is a regulated form of necrosis that depends on receptor-interacting protein kinase (RIPK)3 and mixed lineage kinase domain-like (MLKL). While danger-associated molecular pattern (DAMP)s are involved in various pathological conditions and released from dead cells, the underlying mechanisms are not fully understood. Here we develop a fluorescence resonance energy transfer (FRET) biosensor, termed SMART (a sensor for MLKL activation by RIPK3 based on FRET). SMART is composed of a fragment of MLKL and monitors necroptosis, but not apoptosis or necrosis. Mechanistically, SMART monitors plasma membrane translocation of oligomerized MLKL, which is induced by RIPK3 or mutational activation. SMART in combination with imaging of the release of nuclear DAMPs and Live-Cell Imaging for Secretion activity (LCI-S) reveals two different modes of the release of High Mobility Group Box 1 from necroptotic cells. Thus, SMART and LCI-S uncover novel regulation of the release of DAMPs during necroptosis.
- Publisher
- Springer Nature
- Research Division(s)
- Cell Signalling And Cell Death
- PubMed ID
- 30367066
- Publisher's Version
- https://doi.org/10.1038/s41467-018-06985-6
- Open Access at Publisher's Site
https://doi.org/10.1038/s41467-018-06985-6- NHMRC Grants
- NHMRC/1142669, NHMRC/1107149, NHMRC/1105023,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2018-11-20 09:12:53
Last Modified: 2018-11-20 01:08:46