Isolation and propagation of mammary epithelial stem and progenitor cells
Journal Title
Mouse Cell Culture. Methods in Molecular Biology
Publication Type
Book Section
Abstract
Several methods of mammary gland dissociation have been described that utilize a combined strategy of mechanical and enzymatic dissociation to isolate mammary epithelial cells (MECs) from intact tissue (Smalley et al., J Mammary Gland Biol Neoplasia 17:91-97, 2012). Here we detail a robust method that enables the isolation of all major stem and progenitor MEC populations, which has been successfully used to study stem cell behavior when coupled with transplantation and in vitro assays (Shackleton et al., Nature 439:84-88, 2006; Bouras et al., Cell Stem Cell 3:429-441, 2008; Sheridan et al., BMC Cancer 15:221, 2015; Jamieson et al., Development 144:1065-1071, 2017). Furthermore, we outline two prominent methods for culturing MECs for the purposes of ex vivo manipulation or study: 2D feeder layer cultures and 3D Matrigel colony assays. Importantly, all outlined methods retain stem and progenitor cell behaviors and can be used in combination with downstream in vivo, in vitro, or in silico analyses.
Publisher
Humana Press
Research Division(s)
Immunology; Cancer Biology And Stem Cells
PubMed ID
30788829
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2019-03-13 11:54:07
Last Modified: 2019-03-13 01:38:06
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