Analysis of the spatiotemporal development of hematopoietic stem and progenitor cells in the early human embryo
Journal Title
Stem Cell Reports
Publication Type
Journal Article in press
Abstract
Definitive hematopoietic stem cells (HSCs) first emerge in the aorta-gonad-mesonephros (AGM) region in both mice and humans. An ex vivo culture approach has enabled recapitulation and analysis of murine HSC development. Knowledge of early human HSC development is hampered by scarcity of tissue: analysis of both CFU-C and HSC development in the human embryo is limited. Here, we characterized the spatial distribution and temporal kinetics of CFU-C development within early human embryonic tissues. We then sought to adapt the murine ex vivo culture system to recapitulate human HSC development. We show robust expansion of CFU-Cs and maintenance, but no significant expansion, of human HSCs in culture. Furthermore, we demonstrate that HSCs emerge predominantly in the middle section of the dorsal aorta in our culture system. We conclude that there are important differences between early mouse and human hematopoiesis, which currently hinder the quest to recapitulate human HSC development ex vivo.
Publisher
Cell Press
WEHI Research Division(s)
Epigenetics And Development
PubMed ID
30956115
Open Access at Publisher's Site
https://doi.org/10.1016/j.stemcr.2019.03.003
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2019-04-11 12:23:44
Last Modified: 2019-04-11 12:26:26
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