Assessment of gene function of mouse innate lymphoid cells for in vivo analysis using retroviral transduction
- Author(s)
- Seillet, C; Belz, GT;
- Journal Title
- Target Identification and Validation in Drug Discovery. Methods Molecular Biology
- Publication Type
- Book Section
- Abstract
- Retroviral transduction is commonly used to modulate gene expression and is a powerful approach to understand the role of a gene using gain- or loss-of-function strategies. Retroviral vectors can stably integrate non-viral genes into host genomes, providing long-term modulation of gene expression in infected cells and their progeny. Here we describe the generation of retroviral supernatants and the steps to efficiently transduce genes in innate lymphoid cell (ILC) progenitors for subsequent analysis of ILC populations in vivo.
- Publisher
- Humana Press
- Research Division(s)
- Immunology
- PubMed ID
- 30912025
- Publisher's Version
- https://doi.org/10.1007/978-1-4939-9145-7_14
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2019-04-01 10:18:46
Last Modified: 2019-04-01 11:44:13