Macrophages, rather than DCs, are responsible for inflammasome activity in the GM-CSF BMDC model
- Author(s)
- Erlich, Z; Shlomovitz, I; Edry-Botzer, L; Cohen, H; Frank, D; Wang, H; Lew, AM; Lawlor, KE; Zhan, Y; Vince, JE; Gerlic, M;
- Details
- Publication Year 2019-04,Volume 20,Issue #4,Page 397-406
- Journal Title
- Nature Immunology
- Publication Type
- Journal Article
- Abstract
- Inflammasomes are one of the most important mechanisms for innate immune defense against microbial infection but are also known to drive various inflammatory disorders via processing and release of the cytokine IL-1beta. As research into the regulation and effects of inflammasomes in disease has rapidly expanded, a variety of cell types, including dendritic cells (DCs), have been suggested to be inflammasome competent. Here we describe a major fault in the widely used DC-inflammasome model of bone marrow-derived dendritic cells (BMDCs) generated with the cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF). We found that among GM-CSF bone marrow-derived cell populations, monocyte-derived macrophages, rather than BMDCs, were responsible for inflammasome activation and IL-1beta secretion. Therefore, GM-CSF bone marrow-derived cells should not be used to draw conclusions about DC-dependent inflammasome biology, although they remain a useful tool for analysis of inflammasome responses in monocytes-macrophages.
- Publisher
- Springer Nature
- Research Division(s)
- Immunology; Inflammation
- PubMed ID
- 30742078
- Publisher's Version
- https://doi.org/10.1038/s41590-019-0313-5
- NHMRC Grants
- NHMRC/1037321, NHMRC/1105209, NHMRC/1080321, NHMRC/1143976, NHMRC/1101405, NHMRC/1145788, NHMRC/1141466,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2019-03-13 08:04:15
Last Modified: 2019-04-01 10:13:09